FIG. 2.

Effect of Pter treatment on corticosterone, ACTH, and NORA levels, and CRH expression in melanoma-bearing mice. Corticosterone (A), ACTH (B), and NORA (D) levels in circulating plasma, and CRH expression (C) in the hypothalamic PVN (optical density arbitrary units, a.u.) were measured as indicated under the Materials and Methods section. Blood was collected from the tail vein during a 24-h period (tumors were allowed to grow for 35 days; see Fig. 1). All groups were treated as in Figure 1. Data are mean values ± SD of seven to eight different animals. *Significantly different p < 0.01, comparing melanoma-bearing mice (untreated or treated with Pter) versus nontumor-bearing mice; ⁺Significantly different p < 0.01, comparing both melanoma-bearing mice groups. Data obtained from melanoma-bearing mice treated with vehicle were not significantly different from those calculated for the untreated group (not shown). ACTH, adrenocorticotropin hormone; CRH, corticotropin-releasing hormone; NORA, noradrenaline; PVN, paraventricular nucleus. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars