Skip to main content
. 2016 Jun 10;24(17):974–990. doi: 10.1089/ars.2015.6437

FIG. 4.

FIG. 4.

Effect of Pter treatment on nuclear Nrf2 and its transcription activity in melanoma-bearing mice. (A) Expression of different Nrf2-dependent genes in A2058-RFP, MeWo-RFP, and MelJuso-RFP cells, isolated by laser microdissection (as indicated under the Materials and Methods section) from in vivo growing tumors 35 days after tumor inoculation, was compared versus 48 h cultured HEMa-LP cells (*Significantly different p < 0.05). (B) Expression of the same genes was compared in in vivo growing A2058-RFP, MeWo-RFP, and MelJuso-RFP cells treated and untreated with Pter (as in Fig. 1A) (*Significantly different p < 0.05). All data for (A) and (B), expressing fold change (quantitative RT-PCR, see under the Materials and Methods section for calculations), show mean values ± SD for five to six different experiments. No significant differences in expression of the genes displayed were found when control A2058, MeWo, and MelJuso cells and their RFP counterparts were compared under in vitro conditions (not shown). (C) Nuclear accumulation of Nrf2 from in vivo growing A2058-RFP, MeWo-RFP, and MelJuso-RFP cells was measured by Western blotting (no significant differences were found when these data were compared with those found in control A2058, MeWo, and MelJuso cells, not shown) (mean values ± SD for five different experiments, *Significantly different p < 0.01, comparing Pter treatment vs. controls). (D) Effect of Nrf2 overexpression (see under the Materials and Methods section) on the tumor growth of control and Pter-treated (as in Fig. 1A) A2058-bearing mice. Results obtained in these cells transfected with lentiviral vector not harboring any gene (negative control) were not different from control values (not shown). Data are mean values ± SD from six to seven different experiments (*Significantly different p < 0.01, comparing Pter treatment vs. controls; no significant differences were found when A2058/Tet-Nrf2 cells, ± Pter treatment, were compared with control A2058 untreated cells). Nrf2, nuclear factor (erythroid-derived 2)-like 2. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars

HHS Vulnerability Disclosure