FIG. 5.

Chemosensitization effect of GSH depletion (A) or sod1-AS (B) in melanoma cells isolated from Pter-treated mice. Melanoma cells were cultured as in Figure 1C. PAC.PBP (100 ng/ml) was added to the culture flasks 24 h after seeding. DEM (0.1 mM) was added 6 h later, whereas sod1-AS (10 μM) were added 12 h after seeding [complexation with lipofectamine 2000 (Invitrogen) (44) was used to facilitate sod1-AS uptake by the cells]. Data are mean values ± SD for five to six different experiments (*p < 0.01, comparing all experimental conditions vs. controls). DEM, diethylmaleate; PAC.PBP, paclitaxel protein-bound particles; sod1-AS, antisense oligonucleotides anti-sod1. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars