Table 1.
GR Number in Growing Melanoma Cells in Mice Treated With Pter and/or CRC
| A2058 | MeWo | MelJuso | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Treatment | Tumor vol. (mm3) | 103 GR/cell | CRC (ng/ml plasma) | Tumor vol. (mm3) | 103 GR/cell | CRC (ng/ml plasma) | Tumor vol. (mm3) | 103 GR/cell | CRC (ng/ml plasma) |
| None | 1049 ± 267 | 73 ± 6 | 247 ± 36 | 507 ± 166 | 52 ± 4 | 195 ± 35 | 286 ± 69 | 55 ± 5 | 179 ± 37 |
| Pter | 258 ± 66* | 71 ± 5 | 123 ± 29* | 167 ± 53* | 45 ± 6 | 87 ± 17* | 145 ± 35* | 47 ± 4 | 80 ± 15* |
| CRC | 857 ± 185 | 80 ± 7 | 205 ± 48 | 426 ± 116 | 49 ± 7 | 177 ± 29 | 240 ± 94 | 46 ± 6 | 164 ± 41 |
| Pter+CRC | 906 ± 214 | 72 ± 5 | 196 ± 37 | 451 ± 159 | 50 ± 5 | 186 ± 35 | 229 ± 77 | 51 ± 5 | 175 ± 35 |
Melanoma cells stably expressing the red fluorescence protein (RFP) were inoculated, as in Figure 1, and allowed to grow for 35 days. Treatment with Pter (as in Fig. 1) and/or costicosterone (using ALZET minipumps [ALZET Osmotic Pumps] and jugular vein catheterism [following manufacturer's instructions]; the mean rate of infusion was 0.3 μg of CRC/h) started 1 week after tumor inoculation. Treatment of tumor-bearing mice with vehicles (DMSO-ethanol for Pter as indicated under the Materials and Methods section; or polyethylene glycol 400 for CRC) did not significantly affect the rate of melanoma growth compared with controls (not shown). The number of GRs (expressed as binding sites/cell) was not significantly different when 72 h cultured A2058-RFP, MeWo-RFP, or MelJuso-RFP cells were compared with their wild-type A2058, MeWo, or MelJuso cell counterparts (not shown). Data for GR number, tumor volume (Tumor vol.), and CRC (blood samples were obtained at 12 h circadian time, Fig. 2) displayed in this table were obtained 35 days after tumor inoculation. All tumors had 50–70 mm3 of volume on day 7 after inoculation. GR number on day 7 was not significantly different from GR number on day 35 (not shown). Data are mean values ± SD of six to seven different animals.
Significantly different p < 0.01, comparing all groups versus controls (untreated). Data obtained in melanoma-bearing mice treated with vehicle were not significantly different from those calculated for the untreated group (not shown).
CRC, corticosterone; GR, glucocorticoid receptor; Pter, pterostilbene.