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. 2016 Jun 27;6:28572. doi: 10.1038/srep28572

Figure 2. Neuroepithelial stem cells (NESCs) give rise to physiological mature neurons.

Figure 2

(A–C) NESCs differentiated into TUJ1+ neurons, S100β+ (B) and GFAP+ (C) astrocytes at 28 days post-differentiation (pdD28). (D) Quantifications of TUJ1 and GFAP positive cells derived from P15 and P30 NESCs at pdD28 demonstrate that NESCs maintained stable neurogenic abilities after extensive passaging. Data expressed as mean ± s.d (n = 3) (P > 0.05). (E) NESCs developed into NeuN+ neurons at pdD40. (F,G) NESCs-derived neurons expressed the pre-synaptic protein, SYNAPSIN I at pdD46, and the post-synapse marker, PSD-95 at pdD71 in a punctate pattern. (H,I) NESCs differentiated into glutamergic and GABAergic positive neurons at pdD46. (J) The axons regenerated from NESCs could extend up to 2 cm in the length at pdD46. Scale bars: (F,G), 50 μm, the others 100 μm. Blue: DAPI, nuclear staining.