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. 2016 Jun 27;6:28572. doi: 10.1038/srep28572

Figure 4. Single neuroepithelial stem cell (NESC) can self-organize into miniature neural tubes (NTs) and differentiate into CfuPNs and interneurons.

Figure 4

(A) Schematic of the protocol for generating miniature NTs from a single neuroepithelial cell. The drawings were drawn by the author Xiaoqing Zhu.(B) A NESC seeded on one well of 96-well plate. (C) A NESC self-organized into polarized NTs on day 14. (D) Quantification of the percentages of survival colonies and polarized colonies derived from single human NESCs versus the number of inoculated single cells at Day 14 during continual colony assays. Data expressed as mean ± s.d (n = 4). (E–I) The polarized NTs express NESC markers, such as NESTIN, PAX6, SOX2, ZO-1 and SOX1. (J) Single NESC-derived progenies differentiated into TUJ1+ neurons and GFAP+ astrocytes at pdD26. (K,L) Single NESC-derived progenies differentiated into glutamergic (vGLUT1+) and GABAergic (GABA+) positive neurons at pdD35. (M–Q) Single NESC-derived cells differentiated into FOXP2+, TBR1+ and SATB2+ CfuPNs at (M–O) and CB+, CR+, PV+ interneurons (P,Q) at pdD40. Scale bars: 100 μm. Blue: DAPI, nuclear staining.