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. 2016 Jun 27;6:28714. doi: 10.1038/srep28714

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(a) Functional evaluations of cartilage tissue strands showing positive staining of Safranin-O compared to native cartilage (positive control). (b) sGAG content measurement by DMMB assay type in comparison to native cartilage (control). (c) Positive type II collagen and Aggrecan staining compared to isotype IgG antibody (negative control). (d) Real-time PCR analysis of tissue strands demonstrating significant expression of cartilage-specific genes including Sox9, COL2A, and ACAN compared to monolayer-cultured bovine articular chondrocytes (n=3). All data presented as average ± SD unless otherwise stated.