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. 2016 Jun 27;6:28637. doi: 10.1038/srep28637

Figure 8. Introduction of tumor-suppressive miRNAs reduces DDX3 knockdown-promoted sphere formation.

Figure 8

(a) Sphere-forming capability of DDX3-knockdown cells was repressed by tumor-suppressive miRNAs. shLuc, shDDX3 #2 and shDDX3 #3 cells were transfected with negative control (NC) miRNA and specific miRNA mimics of miR-200b, miR-200c, miR-122, miR-145, miR-10b and miR-519a. At 48 h post transfection, cells were subjected to sphere formation assay. Scale bar is equal to 100 μm. (b) The number of formed spheres in miRNA-transfected cells described in (a) was transformed into fold change relative to that of NC-transfected shLuc cells. Experiments were performed at least three times, and the error bar indicates ± 1 s.d. of the mean. Statistical analyses were carried out using t test (*p < 0.05; **p < 0.01).