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. 2016 Jun 27;6:28613. doi: 10.1038/srep28613

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Copyright © 2016, Macmillan Publishers Limited

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hMADS cells were transfected with a Let-7i-5p mimic or a control mimic at day 14 and then differentiated into brite adipocyte and analyzed at day 18. (A) mRNA and miRNA expression was evaluated by RT-qPCR. (B) UCP1 and PLNA protein levels were evaluated by immunoblotting. ERK1/2 was used as a loading control. Histograms show the quantification of the band intensities (n = 2). (C) Immunostaining for UCP1. Results are mean +/− SEM of 6 independent experiments performed on different series of cells. a: p < 0.05. Scale bar: 20 μm.