Supplemental Figure 4.

Anatomical and functional verification of DSAP lesion. DBH positive neurons in the mNTS (A) and VLM (B) in SAP injected rats. DBH positive neurons in the mNTS (C) and VLM (D) in DSAP injected rats. The quantification of DBH neuronal numbers in the mNTS (E) ( p < 0.005), and VLM (F) (p < 0.0001) after the lesion. Student's t-test analysis. (G) 2 h food intake measurements after 2DG (300 mg/kg BW, s.c.) injection in SAP (n = 10) and DSAP (n = 17) rats. 2-way ANOVA analysis with Bonferroni post-hoc analyses applied. Data are mean ± SEM. 10× bright field images. Scale bars = 200 μm.