Figure 6. Effects of GmDGAT2D overexpression on TAG production in rod1 mutant seeds.

(a) Expression of GmDGAT2D in rod1 mutants, as confirmed by semi-quantitative PCR. (b) Total TAGs in GmDGAT2D-expressing Arabidopsis rod1 mutant seeds. *P < 0.05 by Student’s t test (n > 3) for the significant difference. (c) Fatty acid composition of TAGs from Arabidopsis wild-type (Col-0) seeds, rod1, and rod1 expressing GmDGAT2D. Different letters in a group indicate sample with significant differences (p < 0.05) from each other. Data are expressed as mean ± SD from at least three biological duplicates. T3 transgenic Arabidopsis seeds were used for analysis.