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. 2016 Jun 27;6:28734. doi: 10.1038/srep28734

Figure 8. TXNIP mediates the inhibition of ChREBP nuclear translocation by SalA in PA-treated HepG2 cells.

Figure 8

(A,B) The levels of nuclear and cytosolic ChREBP in rat livers were measured by Western blotting. (C) The total protein level of FAS in the rat liver was evaluated by Western blotting. The data are expressed as the mean ± SD (n = 3), **P < 0.01 vs. the control group; #P < 0.05, ##P < 0.01 vs. the HFD group. HepG2 cells were transfected with control siRNA or TXNIP siRNA for 48 h before treatment with SalA (20 μM). Then the transfected cells were exposed to PA (0.5 mM) for another 24 h. The levels of nuclear and cytosolic ChREBP in HepG2 cells were measured by Western blotting (D,E). Cellular lysate FAS protein levels were determined by Western blotting (F). The data are expressed as the mean ± SD (n = 3), **P < 0.01 vs. control siRNA; #P < 0.05, ##P < 0.01 vs. the si-Con + PA group.