Table 1.

Diagnostic techniques used to detect enteric pathogens in 1020 children aged below five years in Kunming, southwest China

Enteric pathogens	Diagnostic technique					Reference	Comments
	Culture	Biochemical	Serological	PCR	Microscopy
Virus
RVA				√		[41]	NoV was divided into GI and GII strains. RVA, NoV, SaV, and As were RNA viruses detected using RT-PCR. Ad was a DNA virus detected using PCR.
NoV				√		[42, 43]
SaV				√
As				√		[44]
Ad				√		[38]
Bacteria
DEC	√			√		[11, 12, 45, 46]	DEC involved EAEC, EPEC, EIEC, EHEC, and ETEC types. All were detected using qPCR.
NTS	√	√	√			[11, 12]
Shigella spp.	√	√	√
Vibrio cholera	√	√	√
Vibrio parahaemolyticus	√	√	√
Aeromonas spp.	√	√	√
Plesiomonas spp.	√	√	√
Protozoa
B. hominis				√		[47]	B. hominis was detected using PCR, whereas Cryptosporidium spp. and Giardia lamblia were detected using nested PCR.
Cryptosporidium spp.				√		[48, 49]
Giardia lamblia				√

(Note: The “√” symbol indicates the diagnostic techniques applied in this study)