TABLE 1.

HCV NS3/4A, NS5A, and NS5B primers for cDNA synthesis and PCR amplification

Name	Primer sequence (5′–3′)	Annealing temp (°C)
GT5a1_NS3/4A_PCR1_For	TGCTCCACCTTGGTAGGCTGACCGG	50
GT5a2_NS3/4A_PCR1_For	TGACCGGAACGTACATTTATGACC	50
GT5a1_NS3/4A_PCR1_Rev	GTGGAACTTTGTCAGTGGGATCCA	50
GT5a1_NS3/4A_PCR2_For	CCTATGGAGACGAAGGTCATCACG	55
GT5a1_NS3/4A_PCR2_Rev	GAGCAGTTCTGGGCAAAACACAT	55
GT6a1_NS3/4A_PCR1_For	GCCTTCTGAGGTTGGGCGCTTGGAC	55
GT6a1_NS3/4A_PCR1_Rev	CACATGTGGAACTTTGTCAGCGG	55
GT6a1_NS3/4A_PCR2_For	TGAGTGGCCTCCCGGTGTCAGC	60
GT6a1_NS3/4A_PCR2_Rev	GGGACTCCTCCAAGCGAGCGCTAAG	60
GT6e1_NS3/4A_PCR1_For	CCTGTTGTGTTCTCGCCTATGGAG	55
GT6e1_NS3/4A_PCR1_Rev	GACATCCTAGCAGGGTATGGAGC	55
GT6e1_NS3/4A_PCR2_For	GCAGACACGGCCGCGTGTGGCGAC	55
GT6e1_NS3/4A_PCR2_Rev	GAGGCATTCTGGCAGAAACATCTG	55
GT6l1_NS3/4A_PCR1_For	TTGAGAGTCGGATCTTGGACGGGCAC	55
GT6l1_NS3/4A_PCR1_Rev	GTGGAACTTTGTGAGCGGCATTC	55
GT6l1_NS3/4A_PCR2_For	TGCTGACACTGCTGCATGTGGAGAC	55
GT6l1_NS3/4A_PCR2_Rev	GTCCATTCCGCCTGGCCCAAGCT	55
GT5a_NS5A_PCR1_For	GACCTAGTMAACCTCCTGCC	50
GT5a_NS5A_PCR1_Rev	TCAAGCAAGTCCTGCCACAC	50
GT5a_NS5A_PCR2_For	TCTCCGACRCACTACGTGCC	50
GT5a_NS5A_PCR2_Rev	TACACAAGATTGTGGTGGCG	50
GT5a_NS5A_PCR2_Rev	GTGACCTTCTTCTGCCT	50
GT6_NS5A_PCR1_For	GCYTTYAAGATCATGAGYGG	48
GT6_NS5A_PCR1_Rev	AGCAAGTCCTCCCACACGGAG	48
GT6_NS5A_PCR2_For	AYCAGTGGATGAAYAGGCT	48
GT6_NS5A_PCR2_Rev	AGCAGGGCCATTAACCACATC	48
GT5a_NS5B_PCR1_For	GCGGCTTCATATTCTTCCATGCC	50
GT5a_NS5B_PCR1_Rev	GGAGTGTTTAGCTCCCAGC	50
GT5a_NS5B_PCR2_For	GACCTTTCGTCAGGGTCATGGT	50
GT5a_NS5B_PCR2_Rev	GGGAGYAAAAAGATGCCTAC	50
GT6_NS5B_PCR1_For	GGCCYGAYTAYAAYCCRC	50
GT6_NS5B_PCR1_Rev	CTACCGAGCHGGYAGCA	50
GT6_NS5B_PCR2_For	TCNTAYAGYTCNATGCC	48
GT6_NS5B_PCR2_Rev	GGAAGCAGAAAGATGCCTAC	48
GT6a_NS5B_PCR1_For	CTCTCCCAATATGGGCCAGGCCYGAYTACAATCCACC	59
GT6a_NS5B_PCR1_Rev	ATGTTGCTAAGGCCGCTCGTCTATCGAGCGGGGAG	59
GT6a_NS5B_PCR2_For	CTCGGAAGCAGAGTCCTATAGCTCWATGCCCCC	59
GT6a_NS5B_PCR2_Rev	CGTCTACCGAGCGGGGAGCAAAAAGATGCCT	59
GT6e_NS5B_PCR1_For	CACTACCTATATGGGCGAGGCCAGATTACAATCCTCC	59
GT6e1_NS5B_PCR1_Rev	GCTTTGGGCTTCCCCCTTACCGAGCGGGAAGAA	59
GT6e2_NS5B_PCR1_Rev	GCTTTAGGCTTCCCGCTTACCGAGCGGGAAGAA	59
GT6e_NS5B_PCR2_For	CTCCGATGCTGGCTCATATAGCTCCATGCCCCC	59
GT6e_NS5B_PCR2_For	CTCCGATGCTGGTTCGTACAGCTCTATGCCCCC	59
GT6e_NS5B_PCR2_Rev	CGCTTACCGAGCGGGAAGAAGGAAGATGCCTAC	59
GT6l_NS5B_PCR1_For	CACTACCTATATGGGCGCGGCCTGATTACAACCCACC	59
GT6l_NS5B_PCR1_Rev	TGGAGTGTTATGCTCCCTGCCTACCGGGCAGGGAGCA	59
GT6l_NS5B_PCR2_For	TCCTATAGCTCCATGCCACC	59
GT6l_NS5B_PCR2_Rev	GGAGCAGGAAGATGCCTAC	59