FIG 2.

Kinetic profiles revealed by the proteome microarray reflect those defined by conventional MA. The average array signal intensities of the top 16 most reactive antigens indicating exposure to tularemia in Spain are shown (the results are analyzed in detail in Fig. 3). Each line represents a different antigen. (A) Results for group 1 patients (n = 40). These patients had acute infection, seroconverted according to the MA titer, and showed an increase in signal intensity by array analysis. (B) Results for group 2 patients (n = 41). These patients were in the late acute phase of infection, were seropositive at both time points according to the MA titer, and showed a modest increase in signal intensity by array analysis. (C) Results for group 3 patients (n = 18). These patients were in the convalescent phase of infection and were seropositive by MA at both time points but did not show an increase in signal intensity by array analysis. (D) Results for group 4 patients (n = 5). These patients had MA titers of 0 at both time points and were tentatively considered to be free of F. tularensis infection. They showed the lowest signals of the Spanish samples. (E) Results for group 7, consisting of U.S. controls. Array data were normalized by dividing the IVTT reaction protein spot intensity by the sample-specific median of the 77 IVTT reaction control spots printed throughout the chip to give the FOC and then taking the base 2 logarithm of the ratio (log₂ FOC). *, antigens identified to be significant in the work of Sundaresh et al. (25).