TABLE 2.

Reactive antigens identified using the Spanish tularemia sera^a

Antigena	Gene	Product	Avg signal intensity (log2 FOC) of Spanish samples:		P value	P_BHb
			Negative for tularemia	Positive for tularemia
FTT0975c		Conserved hypothetical protein	1.468	2.283	0.0014	0.3622
FTT1696c	mopA	Chaperone protein GroEL	0.810	1.985	0.0000	0.0418
FTT1539c		Conserved hypothetical protein	1.025	1.759	0.0038	0.4156
FTT1627c		Hypothetical protein	1.022	1.531	0.0080	0.6150
FTT0077c	sucB	Dihydrolipoamide succinyltransferase component	0.732	1.509	0.0038	0.4156
FTT1738c	kdpB	Potassium-transporting ATPase B chain	0.932	1.386	0.0155	0.9500
FTT0196c	glnA	Glutamine synthetase	0.844	1.229	0.0399	0.9873
FTT0727		Conserved hypothetical protein	0.626	1.196	0.0019	0.3622
FTT1629c		Hypothetical membrane protein	0.706	1.154	0.0325	0.9873
FTT0607	ispG	1-Hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate	0.580	1.067	0.0129	0.8396
FTT1099c		Hypothetical protein	0.690	1.054	0.0266	0.9873
FTT1637c		Hypothetical protein	0.818	1.039	0.0336	0.9873

^aListed are the top 12 of 27 discriminatory antigens shown in Fig. 3B, i.e., antigens to which sera were significantly reactive when sera from Spanish tularemia cases were compared with sera from Spanish controls by t test (P < 0.05) and were recognized by sera from the tularemia cases with a signal (log₂ FOC) of >1.

^bP_BH, P values corrected for false discovery using the Benjamini-Hochberg method (31).

^cProteins identified to be significant by Sundaresh et al. (25).