TABLE 4.
Performance of TaqMan array card serotyping method on nasopharyngeal specimens
| Quellung serotype or culture resulta | TaqMan array card |
|||
|---|---|---|---|---|
| lytA CT | Serotype result(s) (CT) | DNA (copies/reaction) | Predicted bacterial load from lytA CT (CFU/ml) | |
| 4 | 27 | 4 (29), 23B (30) | 9.33E1 | 9.33E5 |
| 4 | 28 | 4 (28) | 4.70E1 | 4.70E5 |
| 6A | 27 | 6ABCD (29) | 6.77E1 | 6.77E5 |
| 6A | Negative | Negative | NAb | NA |
| 10A | 31 | 10A (33) | 6.49E0 | 6.49E4 |
| 11A | 23 | 11AD (23) | 4.96E2 | 4.96E6 |
| 11A | 28 | 11AD (28) | 5.94E1 | 5.94E5 |
| 11A | 32 | 11AD (35) | 4.16E0 | 4.16E4 |
| 11A | 34 | 11AD (34) | 8.59E−1 | 8.59E3 |
| 17F | 30 | 17F (30) | 1.32E1 | 1.32E5 |
| 17F | 33 | 17F (36) | 1.66E0 | 1.66E4 |
| 19A | 29 | 19A (31), 6CD (33) | 1.79E1 | 1.79E5 |
| 19A | 28 | 19A (30) | 3.71E1 | 3.71E5 |
| 19F | 29 | 19F (29) | 2.85E1 | 2.85E5 |
| 19F | 28 | 19“F” (30) | 4.20E1 | 4.20E5 |
| 19F | 30 | 19F (30) | 1.41E1 | 1.41E5 |
| 19F | 28 | 19F (28) | 5.04E1 | 5.04E5 |
| 19F | 35 | Negative | 3.86E−1 | 3.86E3 |
| 23F | 28 | 23F (31) | 3.63E1 | 3.63E5 |
| 23F | 27 | 23F (28) | 7.45E1 | 7.45E5 |
| 23F | Negative | Negative | NA | NA |
| 35F | 35 | Negative | 3.16E−1 | 3.16E3 |
| NT | 27 | 4 (30) | 3.08E1 | 3.08E5 |
| Pneumococcal culture negative | ||||
| With positive TAC result (n = 1) | 34 | Negative | 9.62E−1 | 9.62E3 |
| With negative TAC result (n = 4) | Negative | Negative | NA | NA |
a
The Quellung reaction was performed with pure culture colonies from the same nasopharyngeal samples. NT, not typeable.
b
NA, not applicable.