TABLE 5.
Diagnostic sensitivity and specificity of the multiplex RT-PCR assay compared to virus isolation for detection of viruses in clinical serum samples
| Sample | No. of samples |
||
|---|---|---|---|
| Total | Positive by: |
||
| RT-PCRa | Virus isolation | ||
| DENV-1 positive | 4 | 4 | 4 |
| DENV-2 positive | 5 | 5 | 5 |
| DENV-3 positive | 5 | 4 | 5 |
| DENV-4 positive | 5 | 5 | 5 |
| DENV negative | 20 | 0 | 0 |
| CHIKV positive | 40 | 40 | 40 |
| CHIKV negative | 10 | 0 | 0 |
a
In comparison with the results of the reference method (virus isolation), the multiplex RT-PCR assay had 95% sensitivity and 100% specificity for DENV and 100% agreement for CHIKV.