Figure 2.

Coculture of RA synoviocytes with immune cells increases Amigo2 expression in both cell types. RA synoviocytes were cocultured with PBMC from healthy donors in the presence or not of PHA for 24 h. In the cocultures, PBMC were separated from synoviocytes by EDTA addition prior cell lysis. Amigo2 expression was assessed by quantitative real-time PCR and was expressed as fold changes compared to synoviocytes cultured alone and exposed to vehicle (A,B). Amigo2 expression was evaluated in both synoviocytes (A) and PBMC (B) cultured alone or together. The production of IL-17A (C) and TNF-α (D) by the cocultures was quantified by ELISA. The production of these cytokines was not detectable in synoviocytes cultured alone. Data are the mean of at least three independent experiments ± SEM. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.