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. 2016 Jun 27;7:254. doi: 10.3389/fimmu.2016.00254

Figure 4.

Figure 4

Amigo2 expression is regulated by MAPKs and HMGB1. RA synoviocytes were preexposed to MAPKs inhibitors for 1 h followed by the addition of a combination of IL-17A and TNF-α (A) or co-exposed to HMGB1 and the TNF/IL-17A combination (B). After 12 h, Amigo2 expression was assessed by quantitative real-time PCR and is expressed as fold changes compared to control situation. SP6000125, JNK inhibitor; SB203580, p38 inhibitor; U0125, ERK inhibitor; Ctl, control; Cyt, cytokines; H, HMGB1. Data are the mean of at least three independent experiments ± SEM. #Comparison with control situation, *comparison with cytokine-treated cells. *,#P ≤ 0.05, **P ≤ 0.01.