Fig. 2.

Expression pattern of KNOX4 in M. truncatula. (A) KNOX4 expression profile in different plant organs. (B and C) In situ hybridization analysis of KNOX4 mRNA in immature seed coat of the wild-type with antisense probe (B) and sense probe (C). (Scale bar, 10 µm.) (D) KNOX4 promoter-GUS expression in immature (Left) and mature (Right) seed coat. (Scale bar, 0.2 cm.) (E and F) KNOX4 promoter-GUS expression in immature seed coat (E) and mature palisade cells of seed coat (F). Brackets B, C, and E indicate epidermis of immature seed coat that will develop into palisade layer (F) in mature seed coat. (Scale bar, 10 µm.)