Fig. 5.

Characterization of CYP86A mutants in M. truncatula. (A) Phylogenetic analysis of CYP86A gene family members in M. truncatula and Arabidopsis. CYP86A_Medtr8g030590 is indicated with an asterisk. The scale bar indicates the sequence divergence is 0.05 per unit bar. (B) Transcript level of CYP86A_Medtr8g030590 gene in different plant organs. (C) Schematic diagram of gene structure and the two allele sites of CYP86A (Medtr8g030590). Boxes represent exons and lines represent introns. (D) Permeability of cuticles of CYP86A mutants stained with toluidine blue. Seed coats were immersed in 0.05% toluidine blue O for 2 min and rinsed with water. (Left) Seeds from CYP86A heterozygous plants. (Right) Seeds from CYP86A homozygous plants. (Scale bar, 0.5 cm.)