Fig. 1.
UNC-73 and UNC-53 promote anteriorly directed neurite extension. (A) Adult TRN morphologies, visualized by RFP expressed from the mec-17 promoter, in wild-type, unc-73(u908), and unc-53(914) animals. Arrows here and elsewhere point to the premature ends of the neurites. (B) TRN morphologies in wild-type or unc-73(u1013) animals at the second larval stage (Left) and early adult stage (Right). mec-3p::RFP was used to label the TRNs and FLPs in unc-73(u1013) mutants. (C) The percentage of TRN anterior neurites terminating in specific zones (red numbers for ALM, green numbers for AVM, and blue numbers for PLM). The animal body is divided into six zones along the anterior–posterior axis. Zone I occupies the area from the nose to the posterior end of the first pharyngeal bulb; AVM and ALM cell bodies, vulva, PVM, and PLM cell bodies define the boundaries from zone II to zone VI. Determined by a χ2 test, results of unc-53(e2432); unc-73(u1031) were not significantly different (P > 0.05) from those of unc-73(u1031) single mutants, and the difference between unc-53(e2432); sem-5(n2019) and unc-53(e2432) was not significant.