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. 2016 Jun 6;113(25):6880–6885. doi: 10.1073/pnas.1520404113

Fig. 6.

Fig. 6.

Silencing of the Aedae-KR eliminates the aversive behavior elicited by contact with the 1728 kinin analog. (A) Relative gene expression was measured by quantitative RT-PCR (RT-qPCR) in control (dsGFP) and KR-silenced mosquitoes at 2 d postinjection. Aedae-KR expression was reduced significantly (∼52%). Data are shown as the mean of three independent experiments ± SEM. (B) Median time control dsGFP-injected mosquitoes spent in contact with sucrose solution or the kinin analog 1728 at a 1-mM concentration. (C) Median time dsKR-injected mosquitoes spent in contact with sucrose or kinin analog 1728 at a 1-mM concentration. Asterisks denote significant differences (*P < 0.05, ***P < 0.001); ns, not significant. Statistical analyses were performed using GraphPad Prism with an unpaired t test to assess the efficiency of knockdown and a Mann–Whitney u test to evaluate the time in contact assays (B and C). Three independent RNAi experiments were performed.