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Cdc6 protein degradation by SDS is mediated through SCFCdc4. CDC6-prA (WT) or CDC6-prA cdc4-1 (cdc4-1) cells were arrested during mitosis by nocodazole first at the permissive temperature, 26 °C. Cells were then incubated at either 26 °C or 36 °C for 1.5 h, and samples were collected every 15 min with or without SDS treatment. Protein was extracted from each time point and subjected to Western blotting to visualize Cdc6-prA. Anti-Pgk1 antibody was used as a loading control.
