Table S1.
Yeast strains used in this study
| Strain | Genotype | Source |
| RUY508 | MATa bar1 ura3 leu2 his3 trp1 can1-100 | A.E.I. (Brooklyn College, Brooklyn, NY) |
| BCY574 | MATa cdc7-4 trp ura leu ade his | J. Diffley (41) |
| YKK2253 | MATa SIC1-13MYC::HIS3 his3 leu2 met15, ura3 | K.K. (Nagoya City University, Nagoya, Japan) |
| YKK2400 | MATa CLB5-13MYC::HIS3 his3 leu2 met15 ura3 | K.K. |
| RUY267 | MATa CLB5-9MYC::TRP ade2 ura3 leu2 his3 trp1 can1-100 | VAY106 (F. Cross, The Rockefeller University, New York) |
| BCY366 | MATa bar SLD2-9MYC::TRP ADE2 | Present study |
| BCY355 | MATa bar1::hisG ade2 ura3 leu2 his3 trp1 can1-100 | DOM0090 (D. Morgan, University of California, San Francisco) |
| BCY077 | MATa bar1 CDC6-prA::HIS3 ade2 ura3 leu2 his3 trp1 can1-100 | A.E.I. |
| BCY078 | MATa bar1 mck1::KanMX CDC6-prA::HIS3 ade2 ura3 leu2 his3 trp1 can1-100 | A.E.I. |
| BCY461 | MATa CDC6-GFP::HIS3 ura3 leu2 his3 trp1 can1-100 | A.E.I. |
| BCY463 | MATa mck1::KanMX CDC6-GFP::HIS3 ura3 leu2 his3 trp1 can1-100 | A.E.I. |
| BCY234 | MATa bar1 URA3::GAL-CDC6(s) ADE2 | A.E.I. |
| BCY581 | MATa bar1 CDC6-T39A-T368A-prA::HIS3 ade2 ura3 leu2 his3 trp1 can1-100 | A.E.I. |
| BCY615 | MATa bar1 cdc7-4 CDC6-T39A-T368A-prA::HIS3 ade2 ura3 leu2 his3 trp1 can1-100 | Present Study |
| BCY061 | MATa mck1::KanMX ade2 ura3 leu2 his3 trp1 can1-100 | A.E.I. |
| BCY639 | MATa sic1::KanMX CDC6-T39A-T368A-prA::HIS3 ade2 ura3 leu2 his3 trp1 can1-100 | Present study |
| YKK1404 | MATa sic1::KanMX his3 leu2 met15 ura3 | K.K. |
| RUY315 | MATa ORC6-prA::HIS3 ade2 ura3 leu2 his3 trp1 can1-100 | A.E.I. |
| RUY266 | MATa CLB2-9MYC::TRP ade2 ura3 leu2 his3 trp1 can1-100 | VAY100 (F. Cross) |
| BCY101 | MATa bar1 MCK1-9MYC:TRP ade2 ura3 leu2 his3 trp1 can1-100 | A.E.I. |
| BCY282 | MATa cdc4-1 CDC6-prA::HIS3 ADE ade2 ura3 leu2 his3 trp1 can1-100 | A.E.I. |
| RUY004 | MATa bar1 LEU2::ORC6-wt lys2 ade2 ura3 leu2 his3 trp1 can 1 | GW185-3-2 [S. Bell (60)] |
| RUY005 | MATa bar1 LEU2::ORC6-rxl lys2 ade2 ura3 leu2 his3 trp1 can 1 | GW186-3-2 [S. Bell (60)] |
| RUY006 | MATa bar1 LEU2::ORC6-ps lys2 ade2 ura3 leu2 his3 trp1 can 1 | GW187-3-2 [S. Bell (60)] |
| BCY466 | MATa LEU2::ORC6-wt URA3::GAL-CDC6 ade2 ura3 leu2 his3 trp1 can1-100 | Present study |
| BCY467 | MATa LEU2::ORC6-rxl URA3::GAL-CDC6 ade2 ura3 leu2 his3 trp1 can1-100 | Present study |
| BCY468 | MATa LEU2::ORC6-ps URA3::GAL-CDC6 ade2 ura3 leu2 his3 trp1 can1-100 | Present study |
| BCY469 | MATa LEU2::ORC6-wt URA3::GAL-CDC6-T368A ade2 ura3 leu2 his3 trp1 can1-100 | Present study |
| BCY470 | MATa LEU2::ORC6-rxl URA3::GAL-CDC6-T368A ade2 ura3 leu2 his3 trp1 can1-100 | Present study |
| BCY471 | MATa LEU2::ORC6-ps URA3::GAL-CDC6-T368A ade2 ura3 leu2 his3 trp1 can1-100 | Present study |