Fig. S5.

Quantitation of MG-derived hydroimidazolone (MG-H1) adducts as products of bisretinoid photodegradation. (A) MG-adducts in posterior eyecups of agouti Abca4^−/− mice were measured by competitive indirect ELISA. MG can also form from intermediates of glycolysis and fatty acid metabolism. Thus, we selected for bisretinoid photodegradation as the source of MG release by exposing mice to blue light (430 nm), as described in SI Materials and Methods. Control mice were not exposed to blue light. Mean ± SE of two samples assayed in duplicate; four mice/sample. (B) Bisretinoid photodegradation is a source of MG. MG-adducts were measured in ARPE-19 cells that had accumulated A2E and were exposed to 430 nm light (P < 0.05). Quantitation by competitive indirect ELISA. Mean ± SE of five to six samples per group; horizontal bar, P < 0.05, one-way ANOVA and Tukey’s multiple comparison test.