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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Gut. 2015 Mar 23;65(6):914–924. doi: 10.1136/gutjnl-2014-308759

Figure 8. HNF4γ overexpression restores VIL1 expression in MKN28 cells and the effect is potentiated by the down-regulation of NR2F2.

Figure 8

(A) HNF4γ mRNA (by qRT-PCR) and protein levels (by western blot) in MKN28 cells transfected with a control vector (mCherry) or a mCherry-HNF4γ-expression construct. (B) Immunostaining with an HNF4γ-specific antibody in transfected MKN28 cells. Images were taken in a Zeiss Axiophot microscope (20X). (C) Effectiveness of NR2F2 knockdown in MKN28 cells using siRNA. (D) Increased mRNA expression (qRT-PCR) of the intestinal metaplasia marker VIL1, but not TFF2 or TFF3 in MKN28 cells overexpressing HNF4γ or overexpressing HNF4γ and knocked down for NR2F2. All values are shown as means ± SD. Pairwise comparisons, as indicated, were performed by Student’s t-test (* indicates P < 0.05).