FIGURE 2.

AaEaR prevents the differentiation of Entamoeba invadens. E. invadens trophozoites were induced to encyst in LG medium diluted at 47% with 5% ABS, in the presence of AaEaR (230 μg/ml) for 72 h. The trophozoites, precysts and cysts were counted, and the cell percentage was obtained using 100% as the total cell number under the control condition without treatment. Representative images of cells that were stained with Calcofluor White m2r are shown below the graph, and the cysts are more fluorescent than the trophozoites. Two independent experiments were performed in duplicate (n = 4), error bars represent SD. ^∗∗∗p < 0.001.