FIGURE 5.

AaEaR does not induce cell death characteristics. The trophozoites (1 × 10⁴) were treated with AaEaR (230 μg/ml) and MTZ (0.25 μg/ml) for 48 h. An ultrastructure analysis by transmission electron microscopy (A) and the number of vacuoles per cell are shown (B). The data were collected from five images per condition. Amoebas (1 × 10⁴) were treated for 48 h with AaEaR (230 μg/ml) or MTZ (0.25 μg/ml), washed and reincubated in fresh TYI-S-33 medium without treatment, and their proliferation was determined at different times (C). The data are representative of three independent experiments that were performed in duplicate, error bars represent SD. ^∗∗p < 0.01, ^∗∗∗p < 0.001.