Extended Data Figure 8. Effects of (2R,6R)-hydroxynorketamine on synaptoneurosome protein and protein phosphorylation levels.

A single administration of (R,S)-ketamine (KET, 10 mg/kg) or (2R,6R)-hydroxynorketamine (HNK, 10 mg/kg) (a,b), did not alter levels of mTOR or phosphorylated mTOR 1- or 24-hours post-injection in the hippocampus of mice. (b-i), Administration of (R,S)-KET or (2R,6R)-HNK did not alter levels of (c,d), mTOR/phosphorylated mTOR, (e,f), eEF2/phosphorylated eEF2, (g,h), proBDNF/mBDNF, or (i,j), GluA1/GluA2 in the prefrontal cortex of mice. The values for the phosphorylated forms of proteins were normalised to phosphorylation-independent levels of the same protein. Phosphorylation-independent levels of proteins were normalised to GAPDH. Data are means ± S.E.M, and was normalised to the saline-treated control group for each protein. Images are cropped; see Supplementary Fig. 1 for complete blot images *p<0.05 (statistical analyses and n numbers see Supplementary Information Table 1). Abbreviations: eEF2, eukaryotic translation elongation factor 2; GAPDH, Glyceraldehyde 3-phosphate dehydrogenase; mBDNF, mature brain-derived neurotrophic factor; mTOR, mammalian target of rapamycin; proBDNF, pro-brain-derived neurotrophic factor; SAL, saline.