Figure 2.
Characterization of diUb FRET pairs 17 a–g. A) Analytical HPLC and B) MS of Lys6‐linked diUb FRET pair 17 a. C) SDS‐PAGE analysis. D) Emission spectra recorded at λ ex=466 nm. E) FRET efficiencies (E) determined by FLIM.
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