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A) SDS‐PAGE analysis of Lys11‐linked diUb cleavage. OTUD2 was incubated with unlabeled diUb, FRET pair 17 b, or a 1:1 mixture; samples were taken after 10, 30, 60, and 180 min. B) Michaelis–Menten kinetics of TRABID for Lys29‐, Lys33‐ and Lys63‐linked diUb, as determined by the FRET assay.
