Fig. 5.

Investigation of reversion in three HRV1A mutants bearing VP1/2A^pro cleavage site mutations. A 50% confluent monolayer of HeLa cells was transfected using DEAE dextran at 34°C with 1.2 μg of in vitro transcribed HRV1A wt or the indicated mutant RNA. H₂O was used instead of RNA for negative controls. 25 minutes post transfection, cells were washed with PBS and covered with 1% low melting agarose in infection medium. Plaques were visualized with crystal violet 48h or 72h post transfection to visualize viral plaques.