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. 2016 Jun 2;1(8):e85888. doi: 10.1172/jci.insight.85888

Figure 8. Effect of overexpression of dopamine D2 receptor on renal ischemia/reperfusion injury.

Figure 8

Mice were subjected to 45 minutes of bilateral renal ischemia, after which the ureters of both kidneys were retrogradely infused with control adeno-associated virus (CAAV) or dopamine D2 receptor adeno-associated virus (DRD2 AAV). (A) The renal cortex was obtained for mRNA determination of TGF-β, fibronectin 1 (FN1), and collagen type 1a1 (Col1a1) (qRT-PCR). (B) Images of kidney sections stained with H&E showing renal morphology. Scale bar: 200 μm. (C) Blood pressure was measured under anesthesia before starting the ischemia period and 14 days after the induction of ischemia/reperfusion and retrograde ureteral infusion of CAAV or DRD2 AAV. SBP, systolic blood pressure. (D) Serum creatinine was measured from blood obtained prior to euthanasia. n = 3/group. (A and D) *P < 0.05 vs. CAAV, t test. (C) *P < 0.05 vs. all others, 1-way ANOVA followed by Tukey test.