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. 2016 May 9;12(6):1015–1028. doi: 10.1080/15548627.2016.1170256

Figure 6.

Figure 6.

The EIF2AK4-ATF4 pathway is required for in pyocyanin-induced autophagy. (A) Knockdown of EIF2AK4 or ATF4 by RNAi inhibited the numbers of GFP-LC3B puncta in Beas-2B cells infected with WT P. aeruginosa PA14 or treated with pyocyanin (PYO, 0.1 mM). The puncta in each cell were counted (right panel). Data are expressed as mean ± SD of 3 independent experiments. a, P < 0.05 vs. negative control (NC); b, P < 0.05 vs. NC + WT ; c, P < 0.05 vs. NC + PYO. (B and C) Immunoblot analysis of LC3B and SQSTM1 in cells. The blot is representative of 3 experiments. The lower panels show quantification of the ratio of LC3B-II or SQSTM1 to ACTB. a, P < 0.05 vs. NC + WT; b, P < 0.05 vs. NC + PYO.