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. 2016 Apr 28;12(6):976–998. doi: 10.1080/15548627.2016.1166317

Figure 2.

Figure 2.

Pharmacological inhibition of autophagy reduces interstitial fibrosis during UUO. C57Bl/6 mice were divided into 4 groups: 1. sham operation; 2. UUO + saline; 3. UUO + 60 mg/kg chloroquine (CQ); 4. UUO + 30 mg/kg 3-methyladenine (3-MA). The mice were sacrificed at the indicated time points (1 wk and 2 wk) and left kidneys were collected for histological and immunoblot analyses. (A) Representative images of immunoblot analysis of LC3B, SQSTM1, ACTA2, and FN1. ACTB was used as a loading control. (B) Densitometric analysis of LC3B, SQSTM1, ACTA2, and FN1 signals. After normalization with ACTB, the protein signal of the sham was arbitrarily set as 1, and the signals of other conditions were normalized with the sham to calculate fold changes. Data are expressed as mean ± SD. *, P < 0.05, significantly different from the sham group; #, P < 0.05, significantly different from the UUO + saline group. (C) Representative images of Masson trichrome staining. Scale bar: 50 µm. (D) Quantitative analysis of Masson trichrome staining. Data are expressed as mean ± SD. *, P < 0.05, significantly different from the sham group; #, P < 0.05, significantly different from the UUO + saline group.