Figure 4.

Autophagy in proximal tubules is impaired in PT-Atg7 knockout mice during UUO. Floxed control (PT-Atg7 FC) and PT-Atg7 KO mice (littermates or age-matched) were subjected to either sham operation or UUO surgery. The mice were sacrificed at the indicated time points (4 d, 1 wk and 2 wk) and left kidneys were collected for histological and immunoblot analyses. (A) Representative images of immunohistochemical staining of LC3B. Scale bar: 20 µm. (B) Enlarged images of LC3B staining. Scale bar: 20 µm. (C) Representative images of immunoblot analysis of LC3B, SQSTM1, and ATG7. PPIB was used as a loading control.