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. 2016 Apr 28;12(6):976–998. doi: 10.1080/15548627.2016.1166317

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© 2016 Taylor & Francis

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Figure 7. — MTOR activation in proximal tubules during UUO is inhibited by autophagy deficiency in PT-Atg7 knockout mice. Animals and their treatment were the same as described in Fig. 4. (A) Representative images of immunoblot analysis of phospho-RPS6KB1 and RPS6KB1. PPIB was used as a loading control. (B) Densitometric analysis of phospho-RPS6KB1 signals. After normalization with RPS6KB1 and PPIB, the protein signal of PT-Atg7 FC sham was arbitrarily set as 1, and the signals of other conditions were normalized with PT-Atg7 FC sham to calculate fold changes. Data are expressed as mean ± SD. *, P < 0.05, significantly different from PT-Atg7 FC sham group; #, P < 0.05, significantly different from the relevant PT-Atg7 FC group.