Figure 10.

TGFB1-induced apoptosis in primary culture of proximal tubular cells is suppressed by inhibition of autophagy. Primary cells were untreated (control) or treated with 5 ng/ml TGFB1 for 24 h in the absence or presence of 20 μM chloroquine (CQ) or 10 mM 3-methyladenine (3-MA). After treatment the cells were collected for morphological, biochemical and immunoblot analyses. (A, D) Representative images of immunoblot analysis of LC3B and SQSTM1. ACTB was used as a loading control. (B, E) Representative images of phase contrast and fluorescence microscopy showing cellular and nuclear morphology of apoptosis. Scale bar: 200 µm. (C, F) Caspase activity. Data are expressed as mean ± SD. *, P < 0.05, significantly different from control group; #, P < 0.05, significantly different from TGFB1 or Atg7 FC TGFB1 group.