Figure 6.

Increased mitochondrial fission regulates the activity of NFKB and TP53 through the ROS-modulated AKT-IKK-NFKBIA and AKT-MDM2 pathways. (A) Intracellular ROS levels were analyzed by flow cytometry in HCC cells treated as indicated. (B) Western blot analyses for protein levels of AKT, phosphorylated AKT (p-AKT), phosphorylated MDM2 (p-MDM2), phosphorylated IKK (p-IKK) and NFKBIA in HCC cells treated as indicated. (C and D) Western blot analyses for protein levels of DNM1L, AKT, p-AKT, p-MDM2 and TP53 in HCC cells transiently transfected with DNM1L siRNA or expression vector and then followed by treatment with 100 µM H₂O₂ or 20 mM NAC for 12 h as indicated. (E and F) Western blot analyses for protein levels of DNM1L, AKT, p-AKT, p-IKK and NFKBIA or nuclear and cytosolic RELA in HCC cells with treatment as indicated.