Fig. 1.
Chronic activity deprivation decreased intrinsic excitability of cerebellar Purkinje cells (PCs). A: experimental scheme. Network activity totally deprived by treatment of 1 μM tetrodotoxin (TTX) for 2 days in organotypic cerebellar slice culture. Electrophysiological recording was performed at anterior lobule (lobule III–V). B: representative traces (left), bar graphs at +400 pA injection (middle), and plots (right) showing that chronic activity-deprivation decreased intrinsic excitability of PCs. C: bar graph showing that chronic activity deprivation increased current threshold (Ithreshold), but voltage threshold (Vthreshold), membrane capacitance (Cm), and membrane potential (Vm) were not changed. D: representative traces (left) and summarizing graph (right) showing the input resistance (Rin) was decreased after chronic activity deprivation. Black, control; gray, deprived. Asterisks in B marked by post hoc Tukey's test, pairwise comparison followed by 2-way repeated-measures ANOVA; *P < 0.05, ***P < 0.001; n.s., no significance.