Table 1.

Parameters of AP properties and waveform

	Vthreshold, mV	Ithreshold, pA	AP Amplitude, mV	Half-width, ms	Rise Time, ms	First Spike Latency, ms	fAHP, mV	mAHP, mV	Rin, MΩ	Vm, mV	Cm, pF
Control	−40.2 ± 1.0	200.6 ± 10.7	55.3 ± 1.0	0.4 ± 0.01	0.25 ± 0.05	24.6 ± 1.6	10.3 ± 0.8	9.8 ± 0.7	85.8 ± 5.4	−52.9 ± 1.2	241.6 ± 17.2
Deprived	−40.3 ± 1.0	235.0 ± 8.2*	57.8 ± 1.0	0.4 ± 0.01	0.22 ± 0.01	28.8 ± 2.1	9.3 ± 1.0	12.7 ± 0.9*	59.2 ± 4.5***	−50.6 ± 1.4	259.1 ± 10.4

Among active membrane properties, current threshold (I_threshold) was increased under deprived condition, whereas voltage threshold (V_threshold) was not changed (see also Fig. 1). Action potential (AP) waveform, including AP amplitude, half-width, 10–90% rise time, and first spike latency, was monitored. The parameters were not affected by activity deprivation. Medium afterhyperpolarization (mAHP) was increased in deprived neurons, whereas fast afterhyperpolarization (fAHP) was not altered. Among the passive membrane properties [membrane capacitance (C_m), membrane potential (V_m), and input resistance (R_in)], only R_in was changed by activity deprivation. For comparison of the active and passive membrane properties, changes described here are from those shown in Fig. 1, C and D.

^*P < 0.05,

^***P < 0.001.