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. 2016 Apr 12;152(1):40–52. doi: 10.1093/toxsci/kfw065

FIG. 3.

FIG. 3.

Confirmation of Nrf2 deletion in Nrf2 Δ7 or +1 mutations using an Nrf2 activator, CDDO-Im. Single dose of CDDO-Im (30 μmol/kg body weight for 6 h) was orally gavaged to rats. A, Loss of Nrf2 induction by CDDO-Im in the liver of rats with Nrf2 Δ7 or +1 mutations. Both basal and CDDO-Im-inducible Nrf2 protein expressions in wild-type rats were lost in rats with Δ7 or +1 mutations. Keap1 was expressed constantly in all groups. Lamin B and αTubulin were an internal control in the nucleus and 1000 × g supernatant, respectively. B, Quantification of the mRNA levels in the liver of rats with Δ7 or +1 mutations using RT-qPCR. Nrf2 target genes, Ho-1 and Gclm, were upregulated in CDDO-Im-treated wild-type rats, but not in rats with Δ7 or +1 mutation. Keap1 was expressed constantly in all groups. Nrf2 were comparable with those the wild-type rats. Gapdh was used as an internal control. The data represent mean ± SD (n = 3–4). **P < .01. Asterisks without brackets indicate the comparison with vehicle-treated wild-type rats.