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. 2016 Apr 12;152(1):40–52. doi: 10.1093/toxsci/kfw065

FIG. 4.

FIG. 4.

Nrf2-dependent gene induction by CDDO-Im. Microarray analyses were performed using wild-type rats and rats with Nrf2 Δ7 or +1 mutations in the absence or presence CDDO-Im. In this analysis, we defined Nrf2-target genes with 2 criteria. First, we selected genes whose levels were induced more than 2-fold in wild-type rats treated with CDDO-Im compared with that in vehicle-treated wild-type rats. Second, we selected genes downregulated more than 2-fold in CDDO-Im-treated Nrf2 knockout rats compared with CDDO-Im-treated wild-type rats. Number (A) and names (B) of the genes that were induced by CDDO-Im but for which induction was canceled by the Nrf2 Δ7 or +1 mutation. The 187 probes correspond to 106 genes. Fifty-three genes marked by shadowing were in common with the mouse genes to which both Nrf2 and MafG bound in ChIP-seq analyses (Hirotsu et al., 2012). Twenty-three genes with black dots were found to show Nrf2-dependent induction by CDDO-Im in a similar set of analyses in which wild-type and Nrf2 knockout mice were treated with CDDO-Im under the same condition as that used for rats.