Effects of a single dose of AFB1 with CDDO-Im in wild-type rats and Nrf2 knockout rats with Δ7 mutation. A, Schedule of treatment of CDDO-Im and AFB1. Rats were gavaged with CDDO-Im (30 μmol/kg body weight) 3 times every other day at 8 am. Twenty-four hours after the last treatment with CDDO-Im, rats were gavaged with AFB1 (25 μg/100 g body weight). Rats in metabolic cages at days 1 and 7 were sacrificed 24 h after administration of AFB1. B, Groups of genotypes and treatments. Rats (200–265 g on the first day of administration of CDDO-Im) were used. C, Percentages of liver to body weight. D, Serum ALT. E, Urine volume. F, Metabolites of AFB1 in the liver, urine, and serum. The data represent mean ± SD (n = 6–7). *P < .05; **P < .01. Asterisks without brackets indicate the comparison with group 1.