Table 1. Nuclear status of porcine oocytes from COCs in vitro matured with DMSO for the first 24h and then for 20h with TCM-199 (24h DMSO+20h TCM-199 group).
| DMSO (%) | No. oocytes | No. GV (%±SEM) | No. proMI (%±SEM) | No. MI (%±SEM) | No. AI+TI (%±SEM) | No. MII (%±SEM) | No. Abnor. (%±SEM) |
|---|---|---|---|---|---|---|---|
| 0 | 109 | 1(0.7±0.8)a | 3(2.9±2.5)a | 22(21.0±4.6)a | 3(3.3±2.5)a | 77(69.7±4.0)a | 3(2.3±1.6)a |
| 0.1 | 110 | 1(1.1±1.2)a | 0(0.0±0.0)a | 24(21.7±2.1)a | 4(4.0±3.6)a | 77(69.7±4.0)a | 4(3.5±1.5)ab |
| 1 | 113 | 0(0.0±0.0)a | 1(0.8±1.0)a | 38(32.1±7.7)a | 7(5.8±2.6)a | 63(58.1±11.3)a | 4(3.1±2.1)a |
| 2 | 102 | 2(2.0±1.3)a | 0(0.0±0.0)a | 28(26.6±4.7)a | 7(6.6±2.8)a | 60(59.5±3.7)a | 5(5.3±3.6)ab |
| 3 | 99 | 2(2.0±1.4)a | 0(0.0±0.0)a | 24(23.8±2.7)a | 4(4.1±2.3)a | 54(55.3±3.7)a | 15(14.8±3.9)bc |
| 4 | 107 | 4(3.8±3.1)a | 0(0.0±0.0)a | 31(28.8±7.9)a | 12(11.1±2.6)a | 33(30.0±9.4)b | 27(26.3±8.4)c |
Note: Within the same column, different superscripts indicate significant differences (p < 0.05). Abbreviations: GV, germinal vesicle; proMI, prometaphase I; MI, metaphase I; AI+TI, anaphase I and telophase I; MII, metaphase II; Abnor., nuclear abnormality.