Table 2. Nuclear status of porcine oocytes from COCs in vitro matured with DMSO for 44h (24h DMSO + 20h DMSO group).
DMSO(%) | No. oocyte | No.GV (%±SEM) | No.proMI (%±SEM) | No.MI (%±SEM) | No.AI+TI (%±SEM) | No.MII (%±SEM) | No.Abnor. (%±SEM) |
---|---|---|---|---|---|---|---|
0 | 94 | 0(0±0)a | 2(2.0±1.2)a | 18(19.4±2.9)a | 4(4.2±1.1)a | 68(72.4±1.7)ab | 2(2.0±1.2)a |
0.1 | 97 | 1(1.0±1.2)a | 0(0±0)a | 14(14.6±5.6)a | 4(4.2±2.6)a | 77(79.2±7.8)a | 1(1.0±1.2)a |
1 | 82 | 4(4.9±3.8)a | 2(2.5±1.6)a | 18(22.1±5.0)a | 3(3.5±2.5)a | 54(65.8±2.0)ab | 1(1.1±1.4)a |
2 | 89 | 2(2.2±1.4)a | 0(0±0)a | 27(29.6±8.2)a | 7(8.1±1.9)ab | 49(56.1±9.6)bc | 4(4.1±3.1)a |
3 | 94 | 4(4.2±2.6)a | 2(2.1±1.3)a | 26(27.6±3.4)a | 22(23.3±6.9)b | 33(35.2±6.4)c | 7(7.5±2.7)a |
4 | 81 | 4(4.8±0.9)a | 6(7.1±1.8)a | 21(25.2±5.0)a | 10(11.4±7.7)ab | 8(9.5±6.8)d | 32(42.0±19.6)b |
Note: Within the same column, numbers with different superscripts differ significantly (p < 0.05).