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. 2016 Jun 27;11(6):e0158055. doi: 10.1371/journal.pone.0158055

Fig 3. Effects of acetic acid on the expression of myoglobin and GLUT4 in L6 myotube cells.

Fig 3

Total RNA was extracted from untreated L6 myotube cells or those treated with 0.5 mM acetic acid for the indicated time period (A; Mb, B; Slc2a4) or for 5 min after the addition of acetic acid (C; Mb, D; Slc2a4) or 0.5 mM AICAR for 12 hr, and 10 μM compound C for 30 min or 2mM araA for 20min. Real-time PCR analysis was carried out for determination of Mb (A, C, E) and Slc2a4 (B, D, F) mRNA levels in L6 myotube cells. Myoglobin or GLUT4 proteins were analyzed by western blotting on the treatment of 0.5 mM acetic acid for 10 min, 0.5 mM AICAR for 12 hours, and 10 μM compound C for 30 min (G, H) or 2 mM araA for 20 min (I, J). Each bar represents the mean ±SE (n = 3–6). Results were analyzed with one-way ANOVA followed by the Tukey-Kramer post hoc test for multiple comparisons. Groups without the same letter are significantly different (p<0.05).