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. 2016 Jun 27;11(6):e0158055. doi: 10.1371/journal.pone.0158055

Fig 4. Effects of acetic acid treatment on glucose, fatty acid uptake and triglyceride accumulation in L6 myotube cells.

Fig 4

(A)Glucose uptake by L6 cells. Differentiated L6 myotube cells were treated with 0.5 mM acetic acid and 100 nM insulin for 24 and 48 hrs in the medium (50μmol/2ml). Each conditioned medium was collected and measured the concentration of glucose. Amount of glucose uptake was calculated by using the amount of glucose remaining in the medium. (B) Fatty acid uptake by L6 cells. (C) TG accumulation in L6 cells. Differentiated L6 myotube cells were incubated with the medium containing 0.6 μmol palmitic acid (300 μmol/L) for 24 and 48 hrs in the presence or absence of 0.5 mM acetic acid or 0.5 mM AICAR. After the incubation, mediums and cells were collected separately and the concentration of NEFA in the mediums and the concentration of TG in the cells were determined. Each bar represents the mean ±SE (n = 3–4). Results were analyzed with one-way ANOVA followed by the Tukey-Kramer post hoc test for multiple comparisons. Groups without the same letter are significantly different (p<0.05).